Microbiology Procedure
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Transmission Electron Microscopy - TEM

Transmission Electron Microscopy - TEM

Transmission Electron Microscopy - TEM is used to see the fine structure of cells. Ultra-thin sections of the object are prepared, by embedding or freezing the specimen and sectioning it with a diamond or glass knife. Sections are floated in water and picked up on a wire grid. They are stained with a heavy metal (gold or palladium) to make certain part dense, and inserted in the vacuum chamber of the microscope.

A 100,000 volt electron beam is focused on the section and manipulated by magnetic lenses. A photograph prepared from the image may be enlarged with enough resolution to achieve a total magnification of over 20 million times. Objects as small as 1.0 nm may be observed.

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