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Turbidometry

Turbidometry

Turbidometry is measuring the amount of light that passes through a. suspension of microbe with a spectrophotometer or other optical measuring device is also used for estimation of cell mass, since the amount of light absorbed or scattered by the microscope is proportional to its cell density. Spectrophotometer measures absorbancy units (A), defined as follows: A = log(l0/l), where I0 is the intensity of light striking a suspension and I, the intensity of light transmitted by the suspension.

When calibrated against bacterial suspensions of known concentrations, spectrophotometers provide an accurate and rapid way to estimate the dry weight (mass) of bacteria per unit volume of culture. The instrument to be used must be calibrated for the particular bacterium and media being studied by directly determining the number of bacteria in a dense suspension and by measuring Its absorbance A, as well as the absorbance of known dilutions of the suspension.

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