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Microbioloogy in 20th Century

The major interests of microbiologists during the time of Pasteur Were the characterization of infectious agents, study of immunity, prevention and cure of diseases and the analysis of chemical activities of microorganisms. During the 20th century, microorganisms have contributed a great deal to the development of other sciences such as biochemistry, genetics and in general, contemporary, biology. The discovery of cell free alcoholic fermentation by Buchner was the beginning of the use of micro organisms in understanding metabolic processes.

A few years later, similarities were found between microbial and animal nutrition. Vitamins required in traces by animals were found to be chemically identical with the growth factors required by bacteria and yeast. Detailed study of the role of these growth promoting factors (vitamins) showed their indispensable role in cell metabolism.

The use of microorganisms in these studies showed similarities at the metabolic level in all living systems. The first important contact between genetics and microbiology occurred in the early 1940's, where George Beadle and Edward Tatum succeeded in isolating mutants of Neurospora requiring specific growth factors. This opened the way to analyze and understand genetic mutations in biochemical terms. In 1943 Salvador E.Luria and Max Delbruck showed that bacterial mutations can provide a satisfactory basis for the study of genetics.

Soon afterwards, the existence of the mechanism of genetic transfer in bacteria was discovered by J. Lederberg and G. Beadle. An early discovery of great importance that went without much recognition was that of Avery, McLeod and McCarty who in 1944 established that the genetic material in bacteria was Deoxyribonucleic acid (DNA).

Subsequently, James Watson, Francis Crick and others elaborated the chemical nature of the hereditary material in bacteria which today is known to be similar to that in higher organisms. In the 1960's, microorganisms were extensively used to understand the mechanism by which the information stored in the genetic material is transcribed and then translated into proteins. The colon bacterium, Escherichia coli became a common tool of the microbiologists, geneticists and the biochemists in the search for understanding various cellular processes. M. Nirenberg, Hargobind Khorana, R.W. Holley, F. Jacob, J.Monod and a host of others using the microbes have added a great deal of information to our knowledge of the living systems.

Microorganisms are today used to understand the control mechanisms involved in cell division and reproduction. The coming together of microbiology, genetics and biochemistry beginning from the 1940's eventually led to a major revolution in biological sciences and to our understanding of biology in molecular terms.

Today, microbiology has established as a separate distinct branch of biology with close relationship with genetics and biochemistry. It has also developed into a complex subject, since each area in microbiology has developed into a specialized subject such as industrial microbiology, medical microbiology, food microbiology,dairy microbiology, soil microbiology, sanitary microbiology, environmental microbiology etc. In all these disciplines the common interest has remained around the microbes.

New methods of exploiting microbes for useful purposes and control of troublesome species has however, remained to be of basic interest.

In the seventies, we have seen further utilization of the microbe to understand both biology and biological processes and the use of bacteria as cloning vehicles. E. coli and other bacteria have been used to clone specific segments of DNA and to produce chemicals hitherto synthesized by tedious chemical procedures. One such example is the development of a E. coli strain by W. Gilbert and others in 1978 containing the genetic information for the synthesis of insulin. The bacteria have been found to be easy to manipulate but unfortunately, these rapid developments of the seventies have

 

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