PhosphateTest

PhosphateTest

	PhosphateTest - An enzyme, phosphatase is normally found in milk. This enzyme in respect of heat tolerance is similar to the rickettsia, Cxiella burnetii (Q. fever organism) or Mycobacterium bovis (tuberculosis bacterium). It thus may be tested whether this enzyme is eliminated during pasteurization, so that it could be assumed that these two microbes are also not present in the milk.
Milk sample is combined with disodium phenyl-phosphate. After few minutes of incubation, a colour reagent (CQC) is added. If phosphatase is present the contents will become blue in colour. If there is no colour change then it may be assumed that there is no phosphatase present.