| Type
of
Germinant
|
Responsible
Spores
|
Remarks
|
| Non
Nutrient
type
Metal
Ions |
B.megaterium
and
B.Polymzxa
strains |
Most
species
have
a
requiremene
t
of
K+
and
Na+
(
100
mM) |
| Bicarbonate |
Some
clostridium
sps. |
Enhance
germination
rate
in
nutrient
or
complex
media.
In
effective
with
normal
B.
subtilis
spores |
| n-dodecylamine |
all
spore |
Causes
rapid
germination
at
micromolar
concentration
and
in
non
physiological
conditions.
Toxic
to
germinated
spores |
| CaDPA |
Most
spores |
Effective
at
30-40
mM
will
often
germinate
both
dormant
and
damaged
spores |
| Nutrient
Type
l-alanine |
B.cerus,
B.megaterium,
B.subtilis,
B.pumilus
many
others
and
also
clostridia |
Sometimes
replacable
by
l-amino
butyric
acid,l-valine,
cysteine
etc,
levels
very
with
strains |
| Other
amino
acids |
many
bacilli |
Usually
less
active
thatn
l-alanine |
| Purine
ribosides |
B.cereus,
but
not
B.subtilis |
In
decreasing
order
Inosine,Adenosine,Guanosine,Xanthosine |
| Lactate |
C.bifermentans,C.tetani |
Only
clostridial
Spores |
| Sugars |
B.Megaterium,
strains
of
B.subtilis
and
clostridial
sps. |
Few
respond
to
glucose
alone,
replacable
by
other
agents. |
| Enzymic
subtilisin
Lysozome |
B.subtilis
and
some
other
sps.
and
all
spores |
Spores
should
be
first
sensitized |
