Microbiology Procedure
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Agar Block Preparations 

Agar Block Preparations 
These preparations can be more correctly described as agar sandwiched cultures. They are successfully used to see the effect of antibiotics on various bacteria under the phase contrast microscope by Pulvertaft (16) and to study the spore germination by Pulvertaft and Haynes (17). The most useful application of this technique is the microscopic observation of fungi (18). This is because it allows observation of fungal culture in various developing stages without disturbing the growth conditions.

Sporulation in fungi can be distinctly observed by this preparation and therefore, this preparation is routinely used in the identification of an unknown fungi. Thus, this method has diagnostic as well as taxonomic signi­ficance in mycology.

Several modifications are available in the literature for the agar block preparation. In this section we will restrict to only three of them which are used routinely in microbiology. Readers interested in other modifications are requested to go through the review on microculture by Quesnel (3).

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