Measurement of volume or thickness:
Volume of the object can be easily calculated from the linear measurements obtained from above mentioned methods, if the object has regular geometry. However, it is difficult to measure volume when object is of irregular shape. In this case volumes are obtained by means of electronic device like coulter counter. It is not possible to get the volume of any specific particle.
In order to measure volume of specimen, it is essential to measure its th1ckness. Though not the exact, ut reasonably precise measurements may be achieved by fine focus adjustment technique. In this method objective with high numerical aperture (N.A.) and smallest possible depth of field is employed.
