Index >> Microscopy Methods in Microbiology >> Measurements from Photomicrographs

Measurements from photomicrographs:
In this method, image scale or picture scale is used rather than magnification. Therefore, it gives better accuracy in measurements. To calculate image scale we must know the magni­fication by objective (Mo) magnification by eye piece (Me), the camera factor, which is the distance (d) in mm, between the photo-eyepiece and the film plane divided 250 mm and the magnification factor of the tube (F). The age scale (IS) of photomicrograph negative is then calculated by the following formula.