The sequence of steps needed to follow for the satisfactory result are as under. Take a clean grease free and dry slide. Make a circle of about 20 mm diameter with the help of glass marker. Deposit a drop of bacterial suspension to the reverse side of the marking in the centre of the circle. Spread the drop evenly as, thin film using sterile wire loop. A little experience will soon determine the amount and the density of the material required for this purpose. Allow the smear to dry in air at room temperature. Rapid drying can be ensured by warming the slide above the Bunsen flame. However, in some of the special staining techniques, preparation of smear requires extreme care. They are discussed in the relevant sections
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