Refraction and Reflection

Microscopy Methods in Microbiology
Microroscopy Methods in Microbiology Part 2
Microroscopy Methods in Microbiology - Introduction
Observation of Microbes in the Living State
Observation of Microbes in Smears
Dyes and Staining
Useful pH Range and Colour Change of Some Indicator Dyes
Factors Affecting Staining
Microscopy and Micrometry
Microscopy and Micrometry - Introduction
Behaviour of Light
Light Theories
Resolving Power
Refraction and Reflection
Bright Field Microscopy
Use of Bright Field Microscope

Refraction and Reflection

Refraction and reflection:
The nature of medium through which light passes during the microscopy has great bearing on the quality of image production. The velocity of monochromatic light in vacuum differs from that in any other medium. The denser the material slower the velocity of light and the ratio of the velocity in vacuo (c) to the velocity in medium (v), is called the refractive index (n) of the medium. Thus $refractionandreflection$
Light travels normally through media differing in refractive index in an undeviated path.

However, if it passes with an inclined angle (i), through one medium to the another having different refractive index, refraction takes place, so that the direction of propagation will change at the boundary between the two media. During the passage of light from dense medium to light medium, if it strikes at an incident angle greater than a critical value (c), as shown in the diagram 2-3c, the light ray will be reflected back into the dense medium without propagating into the lighter medium. The value of critical angle depends upon the nature of two media involved

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