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Index >> Microscopy Methods in Microbiology >> Microscopy Method in Resolving Power

Microscopy Method in Resolving Power

Microscopy Method in Resolving Power:
The obvious purpose of microscope is to magnify the object, but in addition and essentially, it must also resolve the finer details of the object. Thus, the basic difficulty in designing a microscope is not the magnification, but the ability of lens system to distinguish two adjacent points as distinct and separate. This ability is known a the resolving power of the microscope. The useful magnification of micro­scope is that which gives clear image and need not necessarily the highest magnification. .

The resolving power of a microscope depends upon the wavelength of light and numerical aperture (N.A.). The minimum resolvable distance between two lumi­nous points (v) is given by the following formula where l = Wavelength of light and N.A. = Numerical aperture.
ResolvingPower
Thus, the shorter the wavelength of light used land lower the N.A., greater is the resolving power. Thus, with electron beam as the source of illumination more resolution of object is achieved than with visible light

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