1. Wet mount or Wet Film Preparations
Beyond doubt the simplest form of microculture chamber is the wet mount preparation.They are prepared by enclosing the organisms in a layer of liquid between the slide and cover glass. There is ample room for the microorganisms to move while being observed and the results can be recorded permanently by the photographic attachment to the microscope. The major disadvantage of this preparation is that it dries out very rapidly. This can be minimised to some extent by sealing the edges of the cover glass by means of silicone grease to prevent the evaporation of liquid. Other disadvantage includes, the amount of medium that can be accommodated is very less.
