Denitrifying Bacteria

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Nitrogen Fixation Nitrogen Fixation Part 1 Structure of Nitrogenase Components Fe Protein Mo-Fe Protein Mechanism of Nitrogenase Action Hydrogen Evolution Factors Affecting Nitrogenase Activity Nitrogen Fixation Genes Nitrification Nitrate Reduction Glutamate Synthase Pathway

Denitrifying Bacteria

Denitrifying Bacteria -
Thiobacillus denitrificans carries out chemolithotrophic reduction of nitrate. It can oxidize sulphur as well as H₂S or thiosulphate, which are all converted to sulphate. It is therefore a sulphur oxidizing chemoautotroph. Nitrate is the oxidant.

5S + 6NO^-₃+ 2H₂O ---> 5 SO²₄+ 3N₂+ 4H+

Micrococcus denitrificans is a facultative autotroph which/can grow both aerobically or anaerobically. It can utilize organic compounds or molecular hydrogen as the source of energy.

The organic compounds supply the reductant H₂ for nitrate reduction. Molecular oxygen is the electron '3Cceptor under aerobic conditions. Nitrate functions as the electron acceptor under anaerobic conditions. H₂is used to reduce nitrate.

5H₂+ NO^-₃ + 2H+ --> N₂+ 6H₂O

Pseudomonas denitrificans is the main nitrate-reducing representative of the Pseudomonas family. It can reduce nitrate to molecular nitrogen anaerobically. Reduced NAD and format appear to be the best electron donors. Intact cells have been found to reduce NO to N2p. The reduction of N₂O to N₂ occurs only in the presence of lactate as the hydrogen donor.

Paeruginosa can reduce nitrate to nitrogen.

Pseudomonas stutzeri has been found to reduce nitrite.

Bacillus licheniformiis (Enterobacteriaceae) can carry out anaerobic growth with sugars and nitrate (denitrification).

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Nitrogen Fixation Part 1 Ammonification Denitrification Denitrifying Bacteria Ecological Importance of Denitrification Nitrogen Assimilation Transamination Glutamate Dehydrogenase (GDH) Glutamate Synthase Pathway Glutamine Synthetase (GS) Reaction Glutamate Synthase (GOGAT) Reaction Transaminase Reactions Glutamine Synthtase Adenylylation and Deadenylylation

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