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Nitrification

Nitrification -

The ammonia released by ammonification can be used as a source of nitrogen by plants. It is, however, unstable in the ecosystem, and disappears when the pH is alkaline. Therefore, in order to return nitrogen to the food chain, ammonia is converted into nitrate, the major form of nitrogen in the soil. This process of oxidation of ammonia to nitrate is called nitrification. Nitrification ensures a supply of a stable form of nitrogen that can be directly used by green plants.

Nitrification is carried out in two stages by two specific groups of organisms of the family Nitrobacteriaceae. These are strict autotrophs, and obtain their energy from the oxidation of ammonia to nitrite and of nitrites to nitrates. The first step, the oxidation of ammonia to nitrite, is called nitrosification, or nitrite formation. The second step, the oxidation of nitrite to nitrate, is called nitrate formation.

The nitrite forming bacteria are called "nitrosobacteria" while the nitrate forming, group is referred to as the 'nitrobacteria". The nitrifying bacteria form a small group consisting of 8 genera.

Nitrosobacteria Nitrobacteria
Nitrite forming bacteria Nitrate forming bacteria
Ammonia oxidizers Nitrite oxidizers
Nitrosomonas Nitrobacter
Nitrosopira Nitrospina
Nitrosolobus Nitrococcus
Nitrosovibrio  

The nitrifying bacteria are small, gram-negative, rod shaped or spherical bacteria. They arc slow growing bacteria, with generation time of about 10-12 hours.

They arc obligate autotrophs which cannot develop in the absence of the specific inorganic energy source. The exception to this rule are some strains of Nitrobacter which can use acetate, as the carbon and energy source. The strains, however, grow much more slowly with acetate than with nitrite.

 

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