There is evidence for non symbiotic nitrogen fixation in tropical forests and plant fallows. Nitrogen accumulation in standing vegetation of plant follows has been estimated to be 38-16S kg of nitrogen per ha, per year. Sugarcane has been grown for centuries in the tropics without addition of nitrogen fertilizer. It has therefore been suggested that nitrogen fixation occurs in association with sugarcane.
Paddy rice has also been grown for centuries in Asia without application of nitrogen fertilizers. Nitrogenase activity has been found to be associated with the roots of rice plants.
Cyanobacteria and photosynthetic bacteria also play an important part in the nitrogen economy of rice plants.
The major source of nitrogen in the salt marsh grass Spartina alterniflora is biological nitrogen fixation associated with the roots. This explains the high productivity of salmarshes, even though the nitrogen input is comparatively low.
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