Microbiology Procedure
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Index >>Prokaryotes Microbes >> Protoplasts

Protoplasts

Protoplasts - Some microorganisms, both eukaryotic and prokaryotic (e.g. yeasts, molds, bacteria, some higher plants) that have a distinct cell wall can at times exist without a cell wall in what is designated as wall less state. The cell membrane and its intact contents are then called a protoplast. Unless the solute concentration of the suspending fluid is osmotically protective i.e. high enough to balance the intracellular osmotic pressure, protoplasts no longer retained by their thick walls, usually burst.

Protoplasts are, therefore, said to be osmotically fragile.An important property of bacterial cell wall is sensitivity to antibiotic penicillin. Penicillin inhibits synthesis of peptidoglycans. Young actively growing Gram positive bacteria are, therefore, sensitivie to penicillin. Thus these bacteria can be made protoplasts by treating them with penicillin. Animal cells, including human cells, are unsusceptible to penicillin, because there are no peptidoglycans there are no peptidoglycans there.

 

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