Comparison
of
the
Component
of
the
Proteins
Synthesis
Systems
of
Prokaryotes
&
Eukaryotes -
| Prokaryotes | Eukaryotes | |
| Transcription | ||
| Template Enzyme Ribonucleotide triphosphates of: |
DNA/RNA template RNA Polymerase A, U, G and C |
DNA template RNA Polymerase A, U, G ,C |
| Translation | ||
| (A) Activation | ||
| Activating enzymes Amino acids Energy Source Metal ions |
Aminoacycl tRNA syntheses About 20 L-forms ATP Mg++ |
Aminoacycl tRNA syntheses About 20 L-forms ATP Mg++ |
| (B)Initiation | ||
Ribosome subunits |
30S and 50S subunits of 70S ribosomes Often polycistronic tRNAmet N-formylmethionine(f met) f met-tRNA met Formyl tetrahydrofolate GTP IF-1 IF-2 -- -- -- IF-3 |
40S and 60S subunits of 80S ribosomes Monocistronic tRNAmet Methionine(Met) Met-tRNAmet No formylation GTP -- (eIF-2 (eIF-2' eIF-2a1 eIF-2a2 eIF-2a3 eIF-3 |
| Elongation factors Aminoacyl tRNAs |
(c)Elongation | |
| EF-T (EF-Tu (EF-Ts EF-G Aminoacyl tRNAs (aa-tRNAs) |
EF-T (EF-Tu (EF-Ts EF-G Aminoacyl tRNAs (aa-tRNAs) |
|
| (D) Termination | ||
| Termination codon Release factors Enzymes |
UAA/UAG/UGA RF-1 RF-2 RF-3 (S factor) Deformylase Aminopeptidase |
UAA/UAG/UGA
-- |