Pure
Culture
Isolation
Methods/Techniques- Studies
of
Koch
and
Pasteur
firmly
established
the
germ
theory
of
disease,
although
these
studies
were
conducted
under
conditions
which
did
not
guarantee
that
pure
cultures
of
the
causative
organisms
were
used.
Secondly,
not
all
scientists
who
began
to
study
micro
organisms
were
as
skillful
as
Pasteur
and
Koch.
Frequently
therefore,
it
was
claimed
that
microorganisms
had
the
capacity
to
change
their
morphological
form
and
their
physiological
function.
This
phenomenon
came
to
be
known
as
the
doctrine
of "pleomorphism",
while
the
opposing
belief
that
microorganisms
have
a
constant
form
and
function
came
to
be
known
as
the
doctrine
of "monomorphism".
