Index >> Replication of DNA >> Polynucleotide Ligase

Polynucleotide Ligase

Replication is a discontinuous process and takes place in short segments called Okazaki fragments. Polynucleotide ligase joins these fragments into high molecular weight DNA.

In mutants which produce defective ligase, the joining of Okazaki fragments is greatly impaired. Polynucleotide ligase seals single-stranded breaks in DNA molecules during chromosome replication and genetic recombination.