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Index >> Replication of DNA >> Template DNA

Template DNA

 

Template DNA
For synthesis of DNA in appreciable quantities the essential requirements are the four deoxyribonucleoside triphosphates and a DNA template. The previously existing DNA on which new DNA is synthesized is called template DNA.

In experiments it has been found that in vitro synthesis of DNA can take place without template DNA, but there is a time lag of five or more hours before synthesis begins. When the DNA template is present, synthesis of DNA begins immediately.

Template DNA can be single-stranded DNA (ssDNA) or double stranded DNA (dsDNA). ssDNA is found naturally in the bacteriophage cPxl74, or can be formed by separating the two strands of normal dsDNA by heating. ssDNA is a more efficient template than dsDNA because the latter has first to undergo unwinding.

Template DNA acts as a 'master copy' on which new DNA is synthesized by complementary Watson Crick base pairing (A-T, G-C). The newly synthesized DNA has the same base composition as the parent double helical template DNA. This indicates that both strands of template DNA are involved in replication.

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