The nodule is then washed in 70% ethyl alcohol for 3 minutes followed by more washings
with sterile water. The nodule is now crushed with a sterile glass rod in a small aliquot of sterile water. Serial dilutions of the suspension are then made so as to obtain sparse and distinct colonies when an aliquot of the appropriate dilution is plated on yeast extract mannitol agar medium. At the end of one week's incubation, distinct colonies of bacteria are picked up and transferred to agar slants for further identification.
Stepwise procedure for isolation of nodule bacteria from root nodules
Effect of seed inoculation with Rhizobium japotlicum (IARI strains, SB6 + SB16) on the yield of soybean (Glycine max); mean performance of 1972, 1973 and 1974 field trials (from V.R. Balasundaram, unpublished data)
| Locations (variety) |
Yield (kg/ha) |
|
Uninoculated |
Inoculated |
% increase in yield due to inoculation |
Delhi
(Bragg) |
1498 |
1883 |
25.7 |
Pantnagar
(Bragg) |
1308 |
1988 |
52.0 |
Bangalore
(Davis) |
1722 |
2442 |
41.8 |
Effect of seed inoculation with rhizobial culture on the yield of various grain legumes in Tarai soil (pH 7.3)
|
Cajanus cajan (arhar)
|
Cicer arietinum
(Bengal gram)
|
Lens culinaris
(lentil)
|
Temperature |
Yield
(q/ha)* |
Per cent increase over uninoculated control |
Yield
(q/ha)* |
Per cent increase over uninoculated control |
Yield
(q/ha)* |
Per cent increase over uninoculated control |
Uninoculated
(control) |
11.3 |
|
10.5 |
|
8.7 |
|
Inoculated with
IARI culture |
13.5 |
19.47 |
12.7 |
20.94 |
11.5 |
32.20 |
40 kg N/ha |
13.2 |
16.82 |
11.8 |
12.38 |
12.1 |
39.10 |
| -lq = 100 kg |
|
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