Enzyme
Linked
Immunosorbent
Assay
ELISA -
ELISA
has
actually
the
same
sensitivity
as
RAST
and
RIA.
However,
it
does
not
require
radioactivity
or
expensive
equipment.
Antigens
or
antibodies
a
attached
to
a
solid
surface,
and
the
coated
surfaces
are
combined
(immunosorbed)
with
the
test
material.
An
enzyme
system
is
then
linked
to
the
complex,
the
remaining
enzyme
system
is
washed
away
and
the
extent
of
enzyme
activity.
measured.
This
indicates
the
presence
of
antigens
or
antibodies
in
the
material An
application
of
ELISA
is
found
in
the
Gonozyme
test
used
to
detect
gonorrhea
agent
(Neisseria
gonorrhoeae)
antigens
in
the
patient.





