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Fluorescent Antibody Technique   - Fluorescent Antibody technique is a slide test performed by combining particles containing antigens with antibodies and a fluorescent dye. When these three complements react, the dye causes the complex to glow an illumination with UV light under a fluorescent microscope. Two commonly used dyes are fluorescein, which emits an apple green glow, and rhodamine that gives off orange red light.

These techniques may be direct or indirect. In .direct method, the dye is linked to known antibody molecules. The antibodies are then combined with particles (may be bacteria) that may contain complementary antigens. The tagged antibodies. accumulate on the particle surface and the particle glows under the microscope. The indirect method is illustrated by the Ff A-ABS diagnostic procedure used for syphilis antibodies in the blood of patient