Index >> Serology >> Radioallergosorbent Test RAST

Radioallergosorbent Test - RAST This test is an extension of the radioimmunoassay. This may be used to detect IgE or other antibodies as well as a variety of small antigens. RAST is commonly known as "sandwich" teachnique. To detect IgE, specific antigens for this antibody are attached to a matrix particle. Serum suspected to contain IgE is then added. Antibody, if present combines on the surface of the particle. Now another antibody, one that reacts with human antibodies, is added.

This antiglobulin antibody carries a radioactive label. The entire complex will, therefore, radioactive if the antiglobulin antibody combines with the IgE. If, IgE is n present, the particles will not show radioactivity.