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Non Symbiotic Nitrogen Fixation

Non Symbiotic Nitrogen Fixation -Winogradsky in Russia and Beijerinck in Holland indepentenly tried to isolate microorganisms which can fix atmospheric nitrogen without symbiotic acid. Winogradsky was the first to succeed and isolated gram positive, sporeforming, anaerobic bacillus known as Clostridum, pasteurianum.

Beijerinck isolated gram negative, non-sporeforming, aerobic pleomorphic coccoid or rod shaped bacteria belonging, to the genus Azotobacter (Azote means nitrogen in French). However, in recent years a large number of organisms have been isolated that fix nitrogen non symbiotically. This has been achieved by cultivating the microorganism in presence of nitrogen labelled with isotopic 15N

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