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Non Symbiotic Nitrogen Fixing Microorganisms

Non Symbiotic Nitrogen Fixing Microorganisms - The Extensive use of the assay procedure utilizing the nitrogenase catalyzed reduction of acetylene to ethylene coupled with the sensitive gas chromatographic analysis proposed by hardy and his associates in 1968 of the Du Pont laboratory in the U.S.A may be regarded as a turning point in the field of biological nitrogen fixation. .

The ease and rapidity with which this technique could be used gave an impetus to many natural ecosystems. The Brailian group of workers headed by Dobereiner showed how Azotobactor paspali could specifically inhabits the roots of the grass Paspalum notatum and fix abundant amounts of nitrogen observation which has later confirmed by the acetylene reduction reaction.

Dobereiner also introduced the concept of ‘associative symbiosis’ or ‘diazotrophic biocoencis’ after the rediscovery of Azospirillum within plant cells of many graminaceous plants. Blue green algae and Azolla received greater attention in developing countries as a nitrogen supplement in rice cultivation

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