Staining Method of Bouin
In this method (28) cover slip impression smear is prepared. Fixation is used to fix the cells to cover glass. The sequence of steps is as follows. Remove agar slab of about 2 mm thickness on which organism is grown. Keep the cover slip in the centre of petri plate and place the agar slab onto it, facing growth side down. Add Bouin’s fixative (S12) into petri plate till the slab immerses in fixative. Keep it for 3 hours.
