Staining Method of Leifson
The procedure described by Leifson (24) is as under. Prepare the smear as earlier described. Place the slide on rack or black surface board. Cover the smear with working staining reagent (S38) for 5-15 minutes. Allow the stain to react till precipitates are observed in staining solution. The principle underlying this is that, alcohol will evaporate faster than water and when alcohol concentration is reduced to 20-25%
