Staining Method of Levaditi
This method is used to detect spirochetes in tissue. This is the modified method (5) of Becker's in which pyridine is used. This method is rapid and gives better result. The sequence of steps is shown below. Fix a small piece (1 mm thick) of tissue by keeping it in 10% formalin for 24 hours. Wash the tissue in water for 1 hour and then place it in 96-98% alcohol for 24 hours for dehydration. Place the tissue in silver nitrate pyridine solution (S56) for 2 hours at room temperature.
