Staining Method of Maneval
Procedure given by Maneval (19) gives very satisfactory results. Place a drop of 1% congo red on a clean glass slide. Mix a loopful of bacterial suspension and spread gently over a slide. Allow it to air dry at room temperature. Flood the smear with Maneval’s stain (S35) and allow it to react for I minute.
Wash with distilled water, air dry and examine under oil immersion objective. Capsules will be seen colourless against red background and bacterial cell will be stained red or reddish brown in colour.
