Staining Method of Robinow,Agar Block,Schaudinns Fixative,Giemsas Stain,Mount in Water,Oil Immersion,Cytoplasm

Staining Methods in Microbiology
Part 1
Nuclear Material Stains
Staining Methods of Robinow
Rapid Method of Robinow
Feulgen Stain
Staining with Acridine Oragne
Metachromatic Granule (Volutin) Stains
Staining with Methylene Blue
Staining Method of Gohar
Staining Method of Albert Modified by Laybourn
Stain For Intracellular Lipid
Staining Method of Burdon
Stain For Polysaccharides
Staining Method of Hotchkiss
Alcian Blue Stain
Special Staining Techniques
Spirochete Stains
Staining Method of Fontana
Staining Method of Rue
Staining Method of Lavadi
Stain For Azotobactor Cysts
Stains For Mycoplasmas
Staining Method of Diens
Staining of Colony
Coverslip preparation
Staining with Cresyl Fast Violet

Staining Method of Robinnow

Staining Method of Robinow

In the technique given by Robinow (2), preparation of smear and fixation is more important. Take out an agar block having thin bacterial growth and fix it with osmic acid vapour for 2-3 minutes. Make an impression smear on cover slip. Air dry. Place the cover slip by keeping smear side down on the surface of warm Schaudinn's Fixative (S40) for 5 minutes to fix the smear. Rinse the cover slip with water and store in 70% ethanol till needed.

Staining of the smear can be done by removing the cover slip from alcohol and washing with water. Place a coverslip in IN HCl at 60°C for 5-10 minutes. Wash the cover slip with tap water three times and rinse with distilled water. Stain for 30 minutes at 37°C with Giemsa's stain (S41) diluted 10 times
with Sorensen's buffer (S42). Rinse and mount in water: Examine immediately, using oil-immersion objective. DNA rich area will be stained (Figure 6-1) and will appear red, while cytoplasm as colourless.

Index >> Staining Methods in Microbiology >> Staining Method of Robinnow

Staining Method of Robinnow