Staining Method of Rue,Fontana,Potassium Permanganate,Cell Surface,oil Immersion,Spirochetes,Formalin,Dye Acid

Staining Method of Rue

	Staining Method of Rue The technique, described by Rue (4) gives better results than the method of Fontana. In this technique, the apparent thickness of organism is increased by application of basic dye which forms precipitates in the presence of mordant like tannic acid, sodium bicarbonate or potassium permanganate and settles onto the cell surface.
Schedule of staining is as follows: Prepare a smear and air dry. Fix the smear with 1% formalin for 1 minute and dry. Place a drop of 5% NaHCO₃ and then add 10 drops of basic fuchsin (S55). Mix well and stain for 3-5 minutes at room temperature (observe visible precipitates). Wash, dry and examine under oil-immersion objective. Spirochetes appear red in colour.
Microbial processes have been used by man since prehistoric times in the preparation of foods, drugs and textiles. The outstanding examples of traditional microbial processes are the production of wine, beer, vinegar, cheese and retting of flax
anberra offers a special education programme in English language. The University provides the following programmes:
	st of English as a Foreign Language . (ToEFL), 550 with a Test of written English (TWE) of 4.5 or a score of6.5 in International English Language Testing System (lELTS).