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Index >> Staining Methods in Microbiology >> Staining Method of Schaeffer and Fulton, Modified by Ashby

Staining Methods in Microbiology
Part 2
Staining Methods in Microbiology Introduction
Simple Stains
Simple Staining Method for Bacteria in Milk (Method of Breed)
Demonstration of Anthrax Bacilli by Simple Staining
Staining of Selection
Negative Stains
Differential Stains
Staining Method of Hucker and Conn
Staining Method of Preston and Morrell
Rapid Method for Gram Stain
Acid Fast Stains
Gram Reaction and Morphology of Some of the Common Bacterial Genera
Ziehl Neelsen Method
Modifications of Zeihl Neelsen Method
Staining Method of Gross
Staining Method of Truant et al
Cytological Stains
Endospore Stains
Staining Method of Scahaeffer and Fusion Modified by Ashby

Staining Method of Schaeffer and Fulton, Modified by Ashby

	Staining Method of Schaeffer and Fulton, modified by Ashby This method is a modified method of Schaeffer and Fulton (10) given by Ashby (11) in which direct heating is replaced by steam heating. The procedure is as under. Fix the smear by minimum use of heat and place the slide on a rim of beaker of boiling water. Please see that the smear side is facing upwards.
When large droplets of water condense on the lower side of the slide, cover the smear with 5% aqueous malachite green solution. Keep the water boiling and allow the stain to react for 1 minute. Wash the slide with cold water. Treat the smear with 0.5% safranin or 0.05% basic fuchsin for 30 seconds. Wash, dry and examine under oil-immersion objective.
Spore will be stained green, while cytoplasm will appear red in colour. If the microscope optics are not very good chromatic aberration may cause the spore and cell coming into focus at different settings of the microscope. Therefore, when the spores are sharply focused, the cells may be virtually invisible and vice versa. Lipid granules are not stained by this technique

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