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Index >> Staining Methods in Microbiology >> Staining Method of White

Staining Method of White

Staining Method of White
This is a combination of positive and negative technique given by White (16). Place a drop of White staining solution -A (S30) on one end of the slide and mix a loopful of bacterial suspension to it. Spread this mixture with the help of another slide as a film of varied thickness. Air dry and fix the smear with heat. Cool the slide.

Flood the slide with 0.5% HC1, drain, blot gently and dry with gentle heat. Stain the slide with White staining reagent-B (S31) for 15-20 seconds. Drain the stain and without washing air dry and examine the smear under oil-immersion objective. Cells appear blue against orange to golden background, while capsule is seen colourless or light violet.

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