Modified Method of Bailey

Modified Method of Bailey

	Modified method of Bailey The method described here is, the method of Bailey modified by Fisher and Conn (23). Prepare the smear as earlier described. Filter solution-A (S₃₆) onto the smear and allow it to react for about 3-5 minutes. Pour off solution-A and, without washing add freshly filtered solution-B (S₃₇). Allow it to react for 7 minutes. Wash carefully with distilled water.
Before slide dries, cover the smear with Ziehls fuchsin (S₁₉) and heat it from below till steam rises for I minute. Rinse the slide with water. Air dry and examine under oil-immersion objective. Flagella and cell will appear red, background precipitates are usually finer and less conspicuous.