Nuclear Material Stains

Nuclear Material Stains

Nuclear material stains
Bacterial cell lacks well organized nucleus as in higher plants and animals. However, structures of varying size and shape and demonstrated with the help of acid-Giemsa and Feuglen stains, which are normally employed to demonstrate nuclear apparatus in other cells. These structures are called as chromatic body, nucleoid, equivalent and even bacterial chromosome

According to Robinow, chromatin bodies normally lie separately in the cytoplasm and all these in bacteria are homogeneous. They contain largely deoxy ribonucleic acid (DNA) combined with protein. Ribonucleic acid (RNA) is also present in the minor quantity. In a given cell, either DNA or RNA is present in the minor quantity. In a given cell, either DNA or RNA is present as a genetic material, but never both Nuclear bodies of bacteria can be differentially demonstrated by staining with Giemsa's solution after the cells have been treated with IN HCI at 60°C to hydrolyze ribonucleic acid.

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