Rapid method for Gram stain
It should be recognized that the time quoted in the methods for staining have little basis in critical examination of the Gram staining results. Many investigators involved in routine diagnostic work have evolved procedures which are highly effective and time saving. A common technique is described here. Take a heat fixed smear and allow the crystal violet solution (S23) to run over the smear and wash it with undiluted Grams iodine (S24) solution.





