Simple Staining Method For Bacteria In Milk Method Of Breed

Staining Methods in Microbiology
Part 2
Staining Methods in Microbiology Introduction
Simple Stains
Simple Staining Method for Bacteria in Milk (Method of Breed)
Demonstration of Anthrax Bacilli by Simple Staining
Staining of Selection
Negative Stains
Differential Stains
Staining Method of Hucker and Conn
Staining Method of Preston and Morrell
Rapid Method for Gram Stain
Acid Fast Stains
Gram Reaction and Morphology of Some of the Common Bacterial Genera
Ziehl Neelsen Method
Modifications of Zeihl Neelsen Method
Staining Method of Gross
Staining Method of Truant et al
Cytological Stains
Endospore Stains
Staining Method of Scahaeffer and Fusion Modified by Ashby

Simple Staining Method For Bacteria In Milk Method Of Breed

Simple staining method for bacteria in milk (method of Breed)
Detection of bacteria in milk sample is difficult due to the presence of fat and protein. Several commonly used stains are, therefore, not serving the purpose. In this method smear is treated with xylene to remove fat and the fixation is done by alcohol.

The sequence of steps to be followed is as under. Place a clean slide over one centimeter square, drawn on a paper and place 0.01 ml of milk sample in the centre of the square. Spread the sample with needle to cover the square. Dry the smear with gentle heat and immerse the slide in xylene or chloroform to remove fat. Fix the smear with 95% alcohol for 3 minutes. Stain the smear with Breed's methylene blue (S20)
for 2 minutes. Wash the smear with 90% alcohol till smear appears faintly blue. Dry in air and examine under oil immersion objective. Bacteria are seen dark blue against light blue background.

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