It is a polymer of C4-fatty acid, but having no glycerol. Fat globules are also known as sudanophilic granules and are found mainly in old cultures. Bacillus cereus produces large sudanophilic bodies even in simple cultural conditions. They are believed to be the reserve source of carbon.
Fat globules are not easily stained by water soluble dye, but can be demonstrated easily using fat soluble dye. Dye used in this technique is more soluble in lipid than in solvent of the staining solution. Therefore, on application of staining solution, dye moves into lipid material of cell and retained there.
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