(4) used this method in combination with the test of nuclease susceptibility and also to demonstrate the differentiation of single and double stranded forms of nucleic acid molecules. Thus, this method has great significance in studying the growth pattern of viruses in the host cells.
For staining, make a cover slip preparation of living cells, tissue cultures or exudates. Without delay, place the preparation in 3% HCI in alcohol for 5 minutes. Rinse the preparation with citrate phosphate buffer (pH 3.8) two times each for 2 minutes. Stain the preparation with 0.01 % acridine orange for 5-10 minutes. Again rinse with buffer as earlier and mount in buffer. Seal with nail varnish and observe under fluorescent microscope.
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