Bring paraffin sections down to water removing the mercuric chloride precipitates. Stain the sections with Mann's methylene blue-Eosin stain (S60) for 12 hours at 370C. Rinse with water and differentiate under microscope in 70% ethanol containing 0.5% orange-G. In modified method given by Ford, sections are treated for 5 seconds with formalin after staining. Dehydrate the sections in alcohol, clear in xylene and mount in Canada balsam.
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