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Calculation of Biochemical Oxygen Demand
BOD5= [(C1-C2) – ((Vt- Ve)/Vt)(C3-C4)] (Vt/Ve) where

C1 is the dissolved oxygen concentration, in milligrams per litre, of the test solution at time zero.
C2 is the dissolved oxygen concentration, in milligrams per litre, of this same solution after five days.
C3 is the dissolved oxygen concentration, in milligrams per litre, of the blank solution (diluent) at time zero.