Microbiology Procedure
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Most Probable Number (MPN)

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Most Propable Number

In this procedure, water in 10 ml, I mi and 0.1 mt amounts is inoculated into lactose broth tubes. The tubes are incubated and coliform organisms may be identified by their production of gas from the lactose.

By referring to a MPN table, a statistical range of the number of coliform may be determined by observing how many broth tubes showed gas. This method does not detect total number of bacteria in the water nor it locate noncoliforms like Salmonella. However it indicates the presence and quantity of coliforms.

 

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