Membrane Filter Method - When the number of indicator organisms in water is very low, direct inoculation on solid media is not practicable and other methods must be used by which large volumes can be examined. Other, methods of examination include membrane filtration and multiple tube test.
Membrane filter techniques are widely used for the enumeration of bacteria from water sample. In this method the bacterial cells are filtered through a membrane as the sample passes through it. The cells along with the membrane are placed on a suitable solid medium. On incubation these cells produce visible colonies which can be counted





